N-ACYLAMINO ACID AMIDOHYDROLASE
Aminoacylase
CAS: 9012-37-7
Molecular Formula: C30H34Cl2N4O
N-ACYLAMINO ACID AMIDOHYDROLASE - Names and Identifiers
N-ACYLAMINO ACID AMIDOHYDROLASE - Physico-chemical Properties
| Molecular Formula | C30H34Cl2N4O |
| Molar Mass | 537.52316 |
| Appearance | Morphological salt-free, lyophilized powder |
| Color | yellow-brown |
| Storage Condition | 2-8°C |
| MDL | MFCD00081285 |
| Physical and Chemical Properties | Chemical properties light red or light yellow freeze-dried powder, soluble in water, the optimum pH value is 7.0-7.5 (pig kidney, amylase) or 7-8 (mirotrase). Stability: pure enzyme can be preserved for a long time after freeze-drying. After toluene is added to the crude enzyme solution, it can be stored in the refrigerator for 1-2 days in a short period of time. Crude enzyme preparation can exist stably at 5 ℃. When pH is 7, the enzyme aqueous solution keeps its activity unchanged at 70 ℃ for 60min, and when pH is less than 5, it is rapidly inactivated at 70 ℃. Requirements for the chemical structure of the substrate: monocarboxyl amino acid; the amino group on the α-carbon atom in the amino acid is acylated, and the hydrolysis speed of the acylation group is different; the chemical structure of the substrate is different from the enzyme of different sources. Enzymatic reaction: N-acylated-L amino acid H2O; fatty acid anion L-amino acid. |
| Use | Use to separate D-type and L-type amino acids. |
N-ACYLAMINO ACID AMIDOHYDROLASE - Risk and Safety
| Hazard Symbols | Xn - Harmful |
| Risk Codes | R36/37/38 - Irritating to eyes, respiratory system and skin. R42 - May cause sensitization by inhalation |
| Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. S36/37 - Wear suitable protective clothing and gloves. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S24 - Avoid contact with skin. |
| WGK Germany | 3 |
| RTECS | BF4890000 |
| FLUKA BRAND F CODES | 3-10-21 |
| HS Code | 35079090 |
N-ACYLAMINO ACID AMIDOHYDROLASE - Upstream Downstream Industry
| Raw Materials | Ammonium sulfate |
| Downstream Products | L-Aspartic acid L-Serine L-Threonine D-Threonine D-Serine |
N-ACYLAMINO ACID AMIDOHYDROLASE - Introduction
Enzyme Commission (EC) Number: 3.5.1.14 ( BRENDA | IUBMB)
Last Update：2022-10-16 17:40:58
N-ACYLAMINO ACID AMIDOHYDROLASE - Vitality definition
1U：That amount of enzyme which catalyzes the hydrolysis of one micromole of N-Acetyl-L-Methionine per hour at 25° C, pH 7.0.
Last Update：2023-08-16 21:32:38
N-ACYLAMINO ACID AMIDOHYDROLASE - Introduction
Acylase 1 is an extremely stable enzyme in the dry state. In solution, the enzyme is stable at high temperatures (70° C) at pH 7, but below pH 5 it is rapidly and irreversibly inactivated. The enzyme shows a high degree of optical specificity toward its substrates. For this reason, it has been used extensively for the resolution of racemic amino acids.
Last Update：2023-08-16 21:32:38
N-ACYLAMINO ACID AMIDOHYDROLASE - Nature
| EPA chemical information | Aminoacylase (9012-37-7) |
Last Update：2024-04-10 22:29:15
N-ACYLAMINO ACID AMIDOHYDROLASE - Production method
pig kidney, amylase or Aspergillus oryzae are used as raw materials, after homogenization and centrifugation to deprecipitate, ammonium sulfate is used for graded precipitation, water dialysis and centrifugation to obtain crude enzyme solution, and then acetone and ethanol are used for graded precipitation and DEAE-cellulose column chromatography purification to obtain the product.
Last Update：2024-04-10 22:29:15
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Multiple SpecificationsSpot supply
Product Name: Acylase Visit Supplier Webpage Request for quotationCAS: 9012-37-7
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
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Raw Materials for N-ACYLAMINO ACID AMIDOHYDROLASE
Downstream Products for N-ACYLAMINO ACID AMIDOHYDROLASE